ywtE [2017-8-11 16:0:3]

dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)

locus: BSU35850

pI: 4.76

mw: 31.44 kDa

protein length: 286 aa

gene length: 858 bp

function: dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)

product: unknown

essential: no

ec: 3.1.3.-

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.2|Biosynthesis/ acquisition of riboflavin/ FAD]

Gene

Coordinates: 3,695,363 → 3,696,223

The protein

Catalyzed reaction/ biological activity

• dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate [Pubmed|26316208]

Protein family

• Cof family (according to Swiss-Prot)
• [SW|HAD superfamily] [Pubmed|26316208]

Structure

• [PDB|1NRW] ([protein|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|PhoC], 39% identity)

Expression and Regulation

Operons

• genes: [gene|9730F7C18AC7CA68D144717851A0C261D9647E31|ywtE]
  description: [Pubmed|22383849]

• genes: [gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]-[gene|9730F7C18AC7CA68D144717851A0C261D9647E31|ywtE]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

  regulation

  • expressed at high salt concentrations ([protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]) [pubmed|18179421]

Biological materials

Mutant

• MGNA-A546 (ywtE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/546 NBRP B. subtilis, Japan]

References

• 26316208